University of Bahrain
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Shortening full-length aptamer by crawling base deletion – Assisted by Mfold web server application

Show simple item record Gopinath, Subash C.B. Lakshmipriya, Thangavel Arshad, M.K. Md Voon, C.H. Adam, Tijjani Hashim, Uda Singh, Harbant Chinni, Suresh V. 2018-07-30T06:59:01Z 2018-07-30T06:59:01Z 2017-06
dc.identifier.issn 1815-3852
dc.description.abstract Systematic Evolution of Ligands by EXponential enrichment (SELEX) is the method to select the specific aptamer against a wide range of targets. For this process, the initial library usually has a length of random sequences from _25 and it reaches over 100 bases. The lengthy sequences have disadvantages such as difficult to prepare, less stable and expensive. It is wise to prefer shorter version of aptamer for a wide range of applications including drug delivery process. It is a common practice to shorten the full-length aptamer by mapping analyses and it is tedious. Here, we used a crawling method to shorten the aptamer by different sequential deletion of bases from both 50 and 30 ends, assisted by Mfold web server application. Two different kinds of aptamer with varied lengths (randomized region of 30 and 74 bases) were desired for this study, generated against Influenza A/Panama/2007/1999 (H3N2) and gD protein of Herpes Simplex Virus-1. It was found that shortening the aptamer length by crawling pattern is possible with the assistance of Mfold web server application. The obtained results resemble the shortened aptamer derived by mapping analyses. The proposed strategy is recommended to predict the shorter aptamer without involving any wet experimental section. en_US
dc.language.iso en en_US
dc.publisher University of Bahrain en_US
dc.rights Attribution-NonCommercial-ShareAlike 4.0 International *
dc.rights.uri *
dc.subject Aptamer
dc.subject Mfold
dc.subject Influenza
dc.subject Herpes
dc.subject Crawling
dc.subject Base deletion
dc.title Shortening full-length aptamer by crawling base deletion – Assisted by Mfold web server application en_US
dc.type Article en_US
dc.volume 23
dc.issue 1
dc.pagestart 37
dc.pageend 42
dc.source.title Arab Journal of Basic and Applied Sciences
dc.abbreviatedsourcetitle AJBAS

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